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The amplified products were run on a 3% agarose gel stained with ethidium bromide and visualized under UV light. Amplification of the normal CCR5 allele produced a 206 bp fragment, while amplification of the mutant allele (CCR5Δ32) produced a 174 bp fragment.Figure 1: Agarose gel showing the CCR5 gene products in samples from a population of SCD patients in the state of Pernambuco.Enter the world of unlimited free Hidden Cam porn, and you are sure to stay with us for hours!Brazil Cupid has connected thousands of Brazilian singles with their matches from around the world, making us one of the most trusted dating sites. Whether you’re looking for a date or the love of your life, find them in a fun and secure environment on Brazil Cupid.
As the Th1 immune response is associated with inflammation, it has been proposed that the CCR5Δ32 allele could confer a selective advantage on patients with SCD because it induces a less efficient Th1 response .
A total of 795 DNA samples from Afro-Brazilian SCD patients between 3 months and 70 years of age (631 Hb SS, 91 Hb SC, 73 Hb S/β thalassemia; 50.4% male) followed up regularly at HEMOPE were analyzed.
The HEMOPE Foundation Ethics Committee approved this study (n° 017/06), and informed consent was obtained from all participants or those legally responsible for them.
The patients were split into a pediatric group (3 months to 17 years old) with 483 individuals and an adult group (18 to 70 years old) with 312 individuals.
An adult control group of 247 DNA samples from healthy blood donors (18 to 61 years old; 82.2% males) from the same geographical region and with ethnic background similar to those of the patients was analyzed for the CCR5Δ32 polymorphism.